rabbit polyclonal anti fibronectin 1 fn1  (Proteintech)

Journal: Cerebral Circulation - Cognition and Behavior

Article Title: A novel gene edited rat model of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL)

doi: 10.1016/j.cccb.2025.100401

Figure Lengend Snippet: Increased FN1 and LTBP-4 in cerebral arterioles of CRHTRA1 rats. Measurement of FN1 expression in pial arteries was conducted using automated threshold intensity quantification. Vessels were captured from regions of interest identified during vessel thickness quantification. Compared to WT rats (A), CRHTRA1 rats showed significantly increased fluorescent intensity of FN1 in pial arteries (B, C). Similarly, compared to WT rats (D), CRHTRA1 rats showed significantly increased fluorescent intensity of LTBP-4 in pial arteries (E, F). Scale bars = 100 µm. Data shown as mean ± SD of vessels imaged from n = 6 CRHTRA1 rats and n = 6 WT rats. * p < 0.05; *** p < 0.001.

Article Snippet: Extracellular matrix (ECM) protein expression was examined using either rabbit polyclonal anti-fibronectin-1 (FN1) (ProteinTech, 15613–1-AP, 1:200) or mouse monoclonal anti-LTBP-4 (R&D Systems, Minneapolis, MN, AF2885, 1:100).

Techniques: Expressing

Journal: iScience

Article Title: Mechanism of hsa_circ_0069443 promoting early pregnancy loss through ALKBH5/FN1 axis in trophoblast cells

doi: 10.1016/j.isci.2024.111608

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-FN1 , Proteintech , Cat#:15613-1-AP; RRID: AB_2105691.

Techniques: Recombinant, Magnetic Beads, cDNA Synthesis, SYBR Green Assay, Membrane, Blocking Assay, Red Blood Cell Lysis, Selection, Methylation, Extraction, Cell Counting, CCK-8 Assay, Software

Journal: Oncology Letters

Article Title: MicroRNA-139 targets fibronectin 1 to inhibit papillary thyroid carcinoma progression

doi: 10.3892/ol.2017.7201

Figure Lengend Snippet: FN1 is a direct target of miR-139 in PTC. (A) Complementary pairings of miR-139 with FN1 wild-type and mutant 3′ UTR reporter constructs are shown. (B, C) Luciferase activities were determined in TPC-1 and HTH83 cells 48 h after co-transfection with the miR-139 mimic or miR-NC and pMIR-FN1-3′-UTR-Wt or pMIR-FN1-3′-UTR-Mut reporter vector. (D, E) Expression levels of FN1 were detected in TPC-1 and HTH83 cells transfected with the miR-139 mimics or miR-NC and untreated cells using RT-qPCR and Western blot analyses. (F, G) mRNA and protein expression levels of FN1 increased in PTC tissues compared with adjacent normal tissues as assessed by RT-qPCR and Western blot analyses. (H) Correlation between miR-139 expression levels and FN1 mRNA levels in PTC tissues was determined using Spearman's correlation analysis. *P<0.05 compared with respective control.

Article Snippet: The primary antibodies used in this study include rabbit anti-human polyclonal FN1 (15613–1-AP; 1:200 dilution; Proteintech, USA) and mouse anti-human monoclonal GAPDH antibody (sc-47724; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA).

Techniques: Mutagenesis, Construct, Luciferase, Cotransfection, Plasmid Preparation, Expressing, Transfection, Quantitative RT-PCR, Western Blot, Control

Journal: Oncology Letters

Article Title: MicroRNA-139 targets fibronectin 1 to inhibit papillary thyroid carcinoma progression

doi: 10.3892/ol.2017.7201

Figure Lengend Snippet: Up-regulation of FN1 rescues the tumour-suppressive effects of miR-139 on PTC cells. (A) FN1 protein was determined in TPC-1 and HTH83 cells transfected with the miR-139 mimic with or without pcDNA3.1-FN1. (B, C) CCK-8 assay and transwell invasion assays were performed in TPC-1 and HTH83 cells transfected with the miR-139 mimic with or without pcDNA3.1-FN1 (magnification, ×200). *P<0.05 compared with respective control.

Article Snippet: The primary antibodies used in this study include rabbit anti-human polyclonal FN1 (15613–1-AP; 1:200 dilution; Proteintech, USA) and mouse anti-human monoclonal GAPDH antibody (sc-47724; 1:1,000 dilution; Santa Cruz Biotechnology, CA, USA).

Techniques: Transfection, CCK-8 Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: miR-206 inhibits FN1 expression and proliferation and promotes apoptosis of rat type II alveolar epithelial cells

doi: 10.3892/etm.2017.4430

Figure Lengend Snippet: Relative expression of FN1 in four groups. (A) Detection of FN1 mRNA expression in AECII was performed by reverse transcription-quantitative polymerase chain reaction (*P<0.05). (B) Detection of FN1 protein expression in AECII performed by immunofluorescence. N1, fibronectin 1; AECII, type II alveolar epithelial cells; nc, group transfected with pcmv-nc; nc-i, group transfected with pgpu6-206; 206, group transfected with pcmv-206; 206-i, group transfected with pgpu6-206.

Article Snippet: Four slides of AECII were incubated with a polyclonal rabbit anti-mouse FN1 antibody (1:100; 15613-1-AP; Proteintech, Rosemont, IL, USA) at 4°C overnight.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Immunofluorescence, Transfection